astra blue  (Santa Cruz Biotechnology)

Journal: Journal of Ginseng Research

Article Title: First-year seasonal growth regulation mechanism underlying storage root thickening in perennial Panax ginseng

doi: 10.1016/j.jgr.2025.09.002

Figure Lengend Snippet: Developmental patterns of 1-year-old P. ginseng storage roots. (A, B) Representative images of stained root cross-section of P. ginseng plants from 0 to 16 WAD, visualized using polarized light microscopy after staining with Safranin-O and Astra Blue. XV: xylem vessel, CZ: cambial cell layer zone, PH, phloem cells, RD: resin duct cells. Scale bar = (A) 100 μm, (B) 200 μm. (C) The numbers of cambial stem cells in the cambial cell layer zone (CZ) (n = 60).

Article Snippet: After dewaxing in HistoClear, the slides were rehydrated and counterstained with 1 % Safranin-O (Sigma, cat. S2255) and 0.8 % Astra Blue (Santa Cruz Biochem, cat. sc-214558A) for 3 min. Safranin-O preferentially stains lignified cell walls (e.g., xylem vessels) red, while Astra Blue stains cellulose-rich tissues blue, enabling the distinction of vascular and storage tissues.

Techniques: Staining, Light Microscopy

Journal: Journal of Inflammation Research

Article Title: Lymphatic Vessels in Chronic Rhinosinusitis

doi: 10.2147/JIR.S436450

Figure Lengend Snippet: The scatter dot plot ( a ) including mean and SD error bars shows the non-significant results of the quantitative analysis of HA by ELISA in the tissue lysates (1 value below detection limit) of CRSwNP (blue), CRSsNP (red), and control (green) patients normalized to a total protein concentration. The scatter dot plot ( b ) including mean and SD error bars shows the results of the quantitative analysis of HA by ELISA in the nasal mucus (1 outlier, no value below detection limit) of CRSwNP (blue), CRSsNP (red), and control (green) patients normalized to a total protein concentration. Outliers are not included. Statistically significant differences can be seen for CRSwNP vs controls (p<0.001) and for CRSsNP vs controls (p<0.05). The scatter dot plot ( c ) including mean and SD error bars shows the non-significant results of the macrophage cell count in the tissue slides of CRSwNP (blue), CRSsNP (red), and control (green) patients normalized to an area of 7 mm 2 . The scatter dot plot ( d ) shows the result of the correlation between HA and macrophages in nasal tissues. Immunohistochemistry ( e ) of HA (photographed with a 20x objective lens) and macrophages (photographed with a 40x objective lens) in CRSwNP, CRSsNP, and control tissues. Staining by Astra blue without degradation of HA by hyaluronidase is displayed in the panels (i), (ii), (iii) and (iv), whereas the panels (v), (vi), (vii) and (viii) show the same tissues after degradation of HA by hyaluronidase. Counterstaining was performed with Nuclear Fast Red. HA was found in both the epithelium (blue arrows) and the stroma (blue arrowheads). The outer epithelium additionally shows staining of mucopolysaccharides other than HA (pink arrows). Staining of macrophages is displayed in the panels (ix), (x), (xi) and (xii). Antigens were stained with Red Alkaline Phosphatase-Substrate, and counterstaining was performed with Harris’ hematoxylin solution. Asterisks indicate statistically significant results. *<0.05, ***<0.001.

Article Snippet: All sections were then covered by a solution consisting of 4% Astra blue and 3% oxalic acid (both from Carl Roth, Karlsruhe, Germany) diluted in distilled water overnight at 60°C.

Techniques: Enzyme-linked Immunosorbent Assay, Control, Protein Concentration, Cell Counting, Immunohistochemistry, Staining